Translational_Unit
Part:BBa_K1641042:Design
Designed by: Pai Li Group: iGEM15_SYSU_CHINA (2015-09-10)
Fusion protein of Cre-EGFP, with RBS at the beginning
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 287
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This Cre-EGFP do not process ssra, and hence much stable in vivo. As a result, the enzymatic activity seems much higher than those with ssra. Yet, a tiny leakage express might happen even if linked to inductive promoter.
Source
Cre from BBa_K1179058 and EGFP from pBI121-EGFP is fused by overlap-PCR; RBS is added via PCR primer.